1·Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体,优化目前的肝细胞永生化。
2·To construct the retroviral expression vector of Human P58.1 gene and study its expression in lymphocytes.
目的构建人P 58.1基因的逆转录病毒表达载体并探讨该基因在淋巴细胞中的表达。
3·Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
4·The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。