1·Objective to isolate neuroepithelial stem cells from the spinal cord neural tube of the embryonic rat and induce them to differentiate into dopaminergic neurons.
目的从胚胎大鼠脊髓神经管中分离神经上皮干细胞并诱导其向多巴胺能神经元方向分化。
2·Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；